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Nicotine‐evoked disassembly of cortical actin filaments in adrenal chromaffin cells
Author(s) -
Cheek Timothy R.,
Burgoyne Robert D.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80022-9
Subject(s) - chromaffin cell , chemistry , microbiology and biotechnology , phalloidin , actin , biophysics , exocytosis , microfilament , stimulation , adrenal medulla , biology , cytoskeleton , endocrinology , biochemistry , secretion , cell , catecholamine
Using rhodamine‐phalloidin staining it was found that actin filaments are concentrated in the cortex of resting chromaffin cells. Cortical actin filaments were disassembled 15 s after stimulation by nicotine and had reassembled 30 s later. Actin filament disassembly following nicotinic stimulation was also detected using the DNase I inhibition assay. Disassembly was independent of external calcium, insensitive to trifluoperazine and was not elicited by high K + , muscarinic agonists or phorbol ester. Disassembly of cortical actin filaments may allow access of secretory granules to exocytotic sites and act in conjunction with a rise in intracellular free calcium to bring about the full secretory response due to nicotinic agonists.