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Analysis of inactive renin by renin profragment monoclonal antibodies
Author(s) -
Gaillard I.,
Fehrentz J.A.,
Simon D.,
Badouaille G.,
Seyer R.,
Castro B.,
Pau B.,
Corvol P.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80020-5
Subject(s) - renin–angiotensin system , monoclonal antibody , chemistry , antibody , plasma renin activity , biochemistry , microbiology and biotechnology , endocrinology , biology , immunology , blood pressure
Two peptides were synthesized, corresponding to the sequences (−19 to −7) and (−26 to −17) of the prorenin prosegment. Monoclonal antibodies were raised to these sequences and used to characterize human plasma inactive renin. Only anti (−19 to −7) reacted with inactive renin, as measured by direct assay or affinity chromatography. The data were used to evaluate two possible inactive renin stuctures: (i) plasma inactive renin is a truncated prorenin lacking the prosegment N‐terminal portion; (ii) its spatial conformation masks the N‐terminal extremity, preventing interaction of this region with specific antibodies.