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Close evolutionary relationship between the chromosomally encoded β lactamase gene of Klebsiella pneumoniae and the TEM β‐lactamase gene mediated by R plasmids
Author(s) -
Arakawa Yoshichika,
Ohta Michio,
Kido Nobuo,
Fujii Yasuaki,
Komatsu Takayuki,
Kato Nobuo
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80014-x
Subject(s) - klebsiella pneumoniae , homology (biology) , transposable element , gene , plasmid , biology , genetics , nucleic acid sequence , peptide sequence , dna , klebsiella , insertion sequence , escherichia coli , microbiology and biotechnology , genome
Sixty‐three percent homology of nucleotide sequence and 67% homology of deduced amino acid sequence were found between the chromosomally encoded β‐lactamase gene of Klebsiella pneumoniae and the TEM β‐lactamase of transposon Tn3. Moreover, 22 out of 24 amino acid residues are identical around the predicted active site. It is therefore suggested that these two kinds β‐lactamases share a common evolutionary origin. The 0.5 kb DNA fragment of the cloned gene hybridized specifically with the chromosomal DNA of all the K . pneumoniae strains tested which had been isolated in Japan, USA and Europe.