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Preferential glutamine uptake in rat brain synaptic mitochondria
Author(s) -
Steib A.,
Rendon A.,
Mark J.,
Borg J.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80013-8
Subject(s) - mersalyl , glutamate receptor , mitochondrion , glutamine , synaptic cleft , neurotransmitter , biochemistry , synaptosome , neurotransmission , chemistry , synaptic vesicle , biophysics , biology , amino acid , receptor , in vitro , membrane , vesicle
Glutamine uptake has been studied in purified rat brain mitochondria of synaptic or non‐synaptic origin. It was taken up by an active saturable transport mechanism, with an affinity two‐times higher in synaptic than in non‐synaptic mitochondria ( K m = 0.45 and 0.94 mM, respectively), V max of uptake was 7‐times higher in synaptic mitochondria ( V max = 9.2 and 1.3per mg protein, respectively). Glutamine transport was found to be inhibited by L‐glutamate (IC 50 = 0.64 mM) as well as thiol reagents (mersalyl, N ‐ethylmaleimide). It is suggested that differential uptake of glutamine in mitochondria of synaptic or nonsynaptic origin may be a major mechanism in the regulation of the synthesis of the neurotransmitter glutamate.