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Identification of yeast 60 S ribosomal proteins crosslinked to rRNA by 2‐iminothiolane
Author(s) -
El-Baradi T.T.A.L.,
Raué H.A.,
Linnekamp M.,
Planta R.J.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81332-6
Subject(s) - ribosomal rna , ribosomal protein , yeast , chemistry , ribosome , biochemistry , microbiology and biotechnology , biology , rna , gene
Saccharomyces carlsbergensis 60 S ribosomal subunits were treated with the hetero‐bifunctional crosslinking agent 2‐iminothiolane and then subjected to mild UV irradiation to introduce protein‐rRNA crosslinks. The major crosslinked products were identified as proteins L2, L3, L5, L19 and L23 of which L5 was found to be crosslinked at a 3–5‐fold higher efficiency than the other four. Several additional proteins were crosslinked to a detectable but much lower extent.