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Cyclic AMP and c‐myc gene expression in PY815 mouse mastocytoma cells
Author(s) -
Le Gros J.,
De Feyter R.,
Ralph R.K.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81329-6
Subject(s) - mastocytoma , gene expression , gene , microbiology and biotechnology , rna , cell cycle , biology , chemistry , biochemistry , in vitro
The possibility was examined that inhibition of growth of PY815 mouse mastocytoma cells by N 6 , O 2' ‐dibutyryladenosine 3',5'‐cyclic monophosphate (DB cyclic AMP) results from inhibition of c‐myc gene expression. Temporary increases in c‐myc RNA which occurred soon after DB cyclic AMP treatment and upon removal of the drug were not consistent with direct inhibition of c‐myc gene expression by DB cyclic AMP. The increases in c‐myc RNA coincided with the passage through, or accumulation of cells in late G 1 ‐ early S phase. It is proposed that cyclic AMP may stimulate c‐myc gene expression which normally occurs only in late G 1 ‐ early S phase in PY815 cells and that cyclic AMP prevents c‐myc expression in cells at other phases of the cell cycle by inhibiting their progression past a cyclic AMP‐sensitive restriction point in early G 1 phase.