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Inhibition of poly(ADP‐ribose) polymerase causes increased DNA strand breaks without decreasing strand rejoining in alkylated HeLa cells
Author(s) -
Moran Michael F.,
Ebisuzaki Kaney
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81300-4
Subject(s) - poly adp ribose polymerase , polymerase , hela , dna , dna polymerase , microbiology and biotechnology , dna repair , dna polymerase ii , dna clamp , biology , chemistry , biochemistry , polymerase chain reaction , in vitro , gene , reverse transcriptase
Treatment of alkylated HeLa cells with 3‐aminobenzamide, an inhibitor of poly(ADP‐ribose) polymerase, increased the number of DNA strand breaks but did not affect the rate of strand rejoining. This suggests that an increase in DNA incision, not a decrease in ligation, results from the inhibition ofpoly(ADP‐ribose) polymerase in cells recovering from DNA damaged by alkylating agents. Poly(ADP‐ribose) DNA strand break DNA repair