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Characterisation of HlyC and mechanism of activation and secretion of haemolysin from E. coli 2001
Author(s) -
Nicaud J.-M.,
Mackman N.,
Gray L.,
Holland I.B.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81272-2
Subject(s) - hemolysin , secretion , mechanism (biology) , chemistry , biophysics , microbiology and biotechnology , biochemistry , biology , philosophy , virulence , epistemology , gene
In this paper the DNA sequence of the cloned hlyC gene from E. coli 2001 is presented. The gene encodes a protein of 20 kDa which is able to activate the 107 kDa polypeptide encoded by hlyA . This gives rise to a haemolytically active protein which differs from the inactive form in stability and by its migration when analysed by polyacrylamide gel electrophoresis under non‐denaturing conditions. We also show that the inactive form is secreted in the presence of the transport functions hlyB and hlyD . This result rules out any role for the hlyC gene product in the transport of HlyA across the inner membrane