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Synthesis of 1,3‐dihydroxy‐ N ‐methylacridone by cell‐free extracts of Ruta graveolens cell suspension cultures
Author(s) -
Baumert A.,
Gröger D.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81266-7
Subject(s) - ruta graveolens , acridone , sephadex , suspension culture , chemistry , alkaloid , chromatography , cell culture , stereochemistry , biochemistry , enzyme , biology , botany , genetics
Cell‐free extracts of Ruta graveolens cell suspension cultures obtained after (NH 4 ) 2 SO 4 precipitation and gel filtration on Sephadex G‐25 catalyze the condensation of N ‐methylanthranilic acid and malonyl‐CoA in the presence of ATP. The reaction product has been identified as 1,3‐dihydroxy‐ N ‐methylacridone. This alkaloid 6 is apparently the key intermediate in the pathway leading to more complex acridone alkaloids.