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Bile acid binding in plasma: the importance of lipoproteins
Author(s) -
Salvioli G.,
Lugli R.,
Pradelli J.M.,
Gigliotti G.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81257-6
Subject(s) - deoxycholic acid , ursodeoxycholic acid , chemistry , chenodeoxycholic acid , bile acid , ultrafiltration (renal) , cholic acid , lipoprotein , chromatography , partition coefficient , very low density lipoprotein , plasma protein binding , cholesterol , intermediate density lipoprotein , biochemistry
Bile acid (BA) hydrophobicity, evaluated by the octanol‐water partition coefficient, decreases along the series deoxycholic acid ‐ chenodeoxycholic acid ‐ hyodeoxycholic acid ‐ ursodeoxycholic acid ‐ cholic acid (CA) ‐ ursocholic acid (UCA). In vitro experiments carried out using dialysis techniques (to determine the maximum BA binding) and ultrafiltration of plasma pre‐incubated with 0.1 mM BA (to assess the distribution of BA between the different lipoprotein fractions) showed that the maximum binding of BA to plasma and lipoproteins follows the same order of hydrophobicity. The fraction not bound to proteins, greater with the hydrophilic BA (UCA and CA), is distributed in the lipoprotein fractions and in particular in high density lipoproteins.