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Early transmembrane events in tumour cell responses observed by stopped‐flow fluorometry
Author(s) -
Utsunomiya Naoko,
Tsuboi Masamichi,
Nakanishi Mamoru
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81251-5
Subject(s) - stopped flow , chemistry , fluorescence spectroscopy , biophysics , transmembrane protein , fluorescence , biology , biochemistry , optics , physics , kinetics , receptor , quantum mechanics , reaction rate constant
Early transmembrane events of tumour cells (mouse myeloma X5563 and lymphoma RDM4) after binding of a monoclonal antibody against mouse MHC antigen and a mitogenic lectin, Con A, were examined by stopped‐flow fluorometry with 3 different fluorescent probes. The results showed that membrane fluidities of the cells increased first after binding of anti H‐2K k monoclonal antibody (11‐4.1), then calcium was released from intracellular stores into the cytoplasma, and lastly calcium influx occurred from the external medium into the cytoplasma. While Con A only induced calcium influx from the external medium into the cytoplasma.