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Energy expenditure for cyclic retention of NH 3 /NH 4 + during N 2 fixation by Klebsiella pneumoniae
Author(s) -
Kleiner D.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81249-7
Subject(s) - klebsiella pneumoniae , energy expenditure , chemistry , energy metabolism , permeability (electromagnetism) , membrane , analytical chemistry (journal) , klebsiella , fixation (population genetics) , nuclear chemistry , environmental chemistry , biochemistry , biology , endocrinology , escherichia coli , gene
The permeability coefficeint for NH 3 through bacterial membranes was determined to be around 2 × 10 −3 cm·s −1 . This value was used to calculate the outward diffusion of NH 3 from intracellular pools and the energy costs for reabsorption as NH 4 + . For an N 2 ‐fixing continuous culture of Klebsiella pneumoniae an energy expenditure of around 4 ATP per NH 3 produced was calculated, thus increasing significantly the energy requirement for N 2 fixation in vivo.