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Light‐induced Fourier transform infrared (FTIR) spectroscopic investigations of the primary donor oxidation in bacterial photosynthesis
Author(s) -
Mäntele W.,
Nabedryk E.,
Tavitian B.A.,
Kreutz W.,
Breton J.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81247-3
Subject(s) - fourier transform infrared spectroscopy , absorbance , chemistry , bacteriochlorophyll , infrared , photochemistry , photosynthesis , infrared spectroscopy , fourier transform , absorption (acoustics) , analytical chemistry (journal) , photosynthetic reaction centre , spectroscopy , materials science , electron transfer , optics , organic chemistry , biochemistry , physics , quantum mechanics , chromatography , composite material
Fourier transform infrared (FTIR) difference spectroscopy of the primary electron donor (P) photo–oxidation has been performed for reaction centers (RCs) and chromatophores of purple photosynthetic bacteria. In the 1800–650 cm −1 spectral region highly reproducible absorbance changes were obtained that can be related to specific changes of individual bond absorption. Several bands in the difference spectra are tentatively assigned to changes of intensity and position of the keto and ester C−O vibrations of the P bacteriochlorophylls, and a possible interpretation in terms of changes of their environment or type of bonding to the protein is given. Small difference bands in the amide I and II region allow only minor protein conformational changes.