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Three‐dimensional structure of renal Na,K‐ATPase determined by electron microscopy of membrane crystals
Author(s) -
Herbert Hans,
Skriver Elisabeth,
Maunsbach Arvid B.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81238-2
Subject(s) - electron microscope , negative stain , crystallography , electron crystallography , membrane , rod , lipid bilayer , chemistry , microscopy , biophysics , materials science , optics , biology , physics , electron diffraction , biochemistry , diffraction , medicine , alternative medicine , pathology
The three‐dimensional structure of Na,K‐ATPase was determined by electron microscopy and image processing. Tilt series of negatively stained membrane crystals were recorded. The projections were analyzed by Fourier methods and the data combined to a 3‐D model. The unit cell contains two rod‐shaped stain‐deficient regions interpreted as αβ‐protomers of Na,K‐ATPase. The rods are related by dyad axes oriented perpendicular to the membrane. Outside the lipid bilayer the rods contact different protein units on the two sides of the membrane.