Premium
Glutathione S ‐conjugates stimulate ATP hydrolysis in the plasma membrane fraction of rat hepatocytes
Author(s) -
Nicotera Pierluigi,
Baldi Carla,
Svensson Sten-Åke,
Larsson Roger,
Bellomo Giorgio,
Orrenius Sten
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81226-6
Subject(s) - glutathione , chemistry , biochemistry , membrane , atp hydrolysis , atpase , glutathione disulfide , hepatocyte , thiol , hydrolysis , enzyme , in vitro
Incubation of a rat hepatocyte plasma membrane fraction with micromolar concentrations of either glutathione disulfide or various glutathione S ‐conjugates resulted in a several‐fold increase in the rate of ATP hydrolysis. This stimulation was further enhanced when the plasma membrane fraction had been pretreated with agents that arylate or oxidize sulfhydryl groups, suggesting that this ATPase activity is modulated by the protein thiol status of the plasma membrane. It is proposed that this newly discovered ATPase may function in the cellular extrusion of both glutathione disulfide and glutathione S ‐conjugates.