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Implication of proteases in the respiration dependent inactivation of the lactose permease of E. coli
Author(s) -
Therisod Hélène,
Ghazi Alexandre,
Shechter Emanuel
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81209-6
Subject(s) - proteases , lactose permease , lactose , phenylmethylsulfonyl fluoride , biochemistry , chemistry , permease , serine , respiration , transporter , biology , enzyme , gene , botany
The lactose permease of E. coli becomes irreversibly inactivated during lactose transport under conditions of high respiratory activity. This inactivation is characterized by a decrease in the steady state of lactose accumulation, a decrease in the influx rate of lactose, and a decrease in the transmembrane electrical potential. We report here that inhibitors of serine proteases (phenylmethylsulfonyl fluoride and N ‐α‐ P ‐tosyl‐L‐lysine chloromethyl ketone) prevent this inactivation, thus implicating proteases in this process.