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Quantitative measurement of the total, peroxyl radical‐trapping antioxidant capability of human blood plasma by controlled peroxidation
Author(s) -
Wayner D.D.M.,
Burton G.W.,
Ingold K.U.,
Locke S.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81208-4
Subject(s) - antioxidant , chemistry , lipid peroxidation , vitamin e , radical , vitamin c , antioxidant capacity , vitamin , blood plasma , biochemistry , food science
Plasma obtained from fasted humans has been analysed for total radical‐trapping antioxidant content by subjecting it to controlled peroxidation using the thermal decomposition of water‐soluble azobis (2‐amidinopropane hydrochloride) at 37°C to produce peroxyl radicals at a known, steady rate. It is found that the total radical‐trapping antioxidant content is rather similar for the 7 subjects that have been tested and, furthermore, it is 10–20‐times larger than the effect attributable to vitamin E alone. Although it is shown that urate and ascorbate augment the contribution from vitamin E, their contributions (21–34 and 0–2%, respectively) still leave 57–73% of the total antioxidant content unaccounted for. Evidence is presented to show that this previously unrecognized large reserve of antioxidant capacity is attributable to the plasma proteins.