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Inhibition of hepatocyte plasma membrane Ca 2+ ‐ATPase activity by menadione metabolism and its restoration by thiols
Author(s) -
Nicotera Pierluigi,
Moore Margo,
Mirabelli Francesca,
Bellomo Giorgio,
Orrenius Sten
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81131-5
Subject(s) - menadione , hepatocyte , chemistry , metabolism , atpase , biochemistry , membrane , enzyme , in vitro
Incubation of isolated rat hepatocytes with cytotoxic concentrations of menadione resulted in inhibition of plasma membrane Ca 2+ ‐ATPase activity. This could be restored by subsequent treatment with either dithiothreitol or reduced glutathione, suggesting that the inhibition by menadione was due to oxidation of sulfhydryl groups critical for Ca 2+ ‐ATPase activity.