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Difference in pathway of Escherichia coli outer membrane permeation between penicillins and cephalosporins
Author(s) -
Yamaguchi Akihito,
Tomiyama Noribumi,
Hiruma Ryoichi,
Sawai Tetsuo
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81130-3
Subject(s) - porin , permeation , bacterial outer membrane , cephalosporin , chemistry , penicillin , membrane , escherichia coli , cephalosporin antibiotic , biophysics , permeability (electromagnetism) , chromatography , microbiology and biotechnology , biochemistry , antibiotics , biology , gene
The differences in the outer membrane permeation between two major subgroups of β‐lactam antibiotics were studied. The permeation of cephalosporins was closely related to porin channels in the outer membrane. In contrast, the outer membrane permeation of penicillins did not decrease in porin‐deficient mutants and, in Rd‐type mutants, their permeability became proportional to the hydrophobicity of the molecules. The activation enthalpy of the penicillin permeation was significantly larger than that of cephalosporins. These observations indicate that penicillins can use the hydrophobic region for the major route of outer membrane passage whereas the cephalosporin permeation is restricted to the pathway via the porin pore.