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Folding of single‐stranded DNA on the histone octamer
Author(s) -
Caffarelli E.,
Leoni L.,
Savino M.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81115-7
Subject(s) - histone octamer , nucleosome , histone , dna , biophysics , chromatosome , linker dna , histone h1 , chemistry , microbiology and biotechnology , biology , biochemistry
A complex between the single‐stranded DNA of the bacteriophage M13 and the histone octamer was analyzed by electron microscopy, low‐angle X‐ray diffraction and nuclease analysis. The morphology and the diffraction pattern of the complex strongly resemble those of the nucleosome. These results, as well as the finding of a protected DNA fragment about 100 nucleotides long following single‐stranded DNA specific nuclease digestion, indicate that ‘a nucleosome‐like’ complex can be formed between single‐stranded DNA and the histone octamer. Competition experiments suggest that under physiological conditions the histone octamer is transferred from single‐ to double‐stranded DNA.