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Prostaglandin E 2 ‐like activity of 20:3 n ‐9 platelet lipoxygenase end‐product
Author(s) -
Lagarde M.,
Burtin M.,
Rigaud M.,
Sprecher H.,
Dechavanne M.,
Renaud S.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81112-1
Subject(s) - lipoxygenase , chemistry , platelet , product (mathematics) , prostaglandin , biochemistry , medicine , enzyme , mathematics , geometry
5,8,11‐Icosatrienoic acid (20:3 n ‐9), a fatty acid associated with platelet hyperactivity, was oxygenated by platelet lipoxygenase. The end‐product of this pathway was purified by high‐performance liquid chromatography (HPLC) and characterized as 12‐hydroxy‐5,8,10‐icosatrienoic acid [12‐OH‐20:3(5,8,10)] by capillary gas‐liquid mass spectrometry. When tested upon platelet aggregation, 12‐OH‐20:3(5,8,10) exhibited a biphasic effect. At low concentrations (below 5 × 10 −7 M) it potentiated aggregation but inhibited it at higher levels, a pattern similar to that obtained with prostaglandin E 2 . However, since the amounts of 12‐OH‐20:3(5,8,10) generated under thrombin stimulation are in the range of concentrations with potentiating effects, it seems that the 12‐OH derivative is responsible for the hyperaggrebility of 20: 3 n ‐9‐rich platelets.