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Association of the glucocorticoid hormone receptor with ribonucleic acid
Author(s) -
Economidis Ioannis V.,
Rousseau Guy G.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81111-x
Subject(s) - glucocorticoid receptor , receptor , rna , chemistry , nuclear receptor , microbiology and biotechnology , uridine , biochemistry , in vitro , biology , transcription factor , gene
The hypothesis that the glucocorticoid hormone receptor interacts with RNA has been tested in cultured rat hepatoma cells. The receptor was covalently labeled with radioactive dexamethasone mesylate, and putative RNA‐receptor complexes were stabilized by either cell‐free crosslinking using formaldehyde or irradiation of intact cells. After chemical cross‐linking in vitro, the receptor displayed the buoyant density of a ribonucleoprotein in CsCl gradients. After photochemical crosslinking in cells labeled with radioactive uridine, the receptor analysed by polyacrylamide gel electrophoresis was carrying labeled ribonucleotides.