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Kinetic analysis of short‐term effects of α‐agonists on gluconeogenesis in isolated rat hepatocytes
Author(s) -
Leverve X.M.,
Groen A.K.,
Verhoeven A.J.,
Tager J.M.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81110-8
Subject(s) - gluconeogenesis , stimulation , glycerol kinase , cytosol , chemistry , transaminase , glycerol , medicine , biochemistry , endocrinology , metabolism , biology , enzyme
Isolated hepatocytes from fasted rats were perifused with glycerol as gluconeogenic substrate. Stimulation of gluconeogenesis with phenylephrine (10 −5 M) as α‐adrenergic agonist consisted of two distinct phases. The first phase was a transient stimulation of gluconeogenesis and was accompanied by transient changes in cytosolic and mitochondrial redox state; this phase was abolished by the transaminase inhibitor aminooxyacetate. The second phase was a stable stimulation of less magnitude, without change in redox state and insensitive to addition of aminooxyacetate. It is concluded that the first phase is due to a transient enhancement of flux through the malate/aspartate shuttle and that the stable phase is probably due to a stimulation of mitochondrial glycerol‐3‐phosphate dehydrogenase and glycerol kinase.