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Isolation and characterization of syncytiotrophoblast plasma membrane from human placenta
Author(s) -
Khalfoun B.,
Degenne D.,
Arbeille-Brassart B.,
Gutman N.,
Bardos P.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81108-x
Subject(s) - syncytiotrophoblast , percoll , membrane , human plasma , centrifugation , chemistry , differential centrifugation , chromatography , human placenta , isopycnic , placenta , density gradient , biochemistry , fetus , biology , pregnancy , genetics , physics , quantum mechanics
Human full‐term syncytiotrophoblast plasma membranes isolated by mechanical procedures (sieving and ultrasonic disintegration), purified by phase centrifugation, form a single band of 1.052 ± 0.002 density in percoll gradient. The purity of the preparation was assessed by electron microscopy, enzyme analysis and β 2 ‐microglobulin determination.