Premium
Pre‐replicative changes of the rat sinusoidal plasma membrane glycoproteins during hepatic regeneration
Author(s) -
Enrich Carlos,
Gahmberg Carl G.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81104-2
Subject(s) - galactose oxidase , glycoprotein , sialic acid , regeneration (biology) , membrane glycoproteins , biochemistry , chemistry , liver regeneration , galactose , neuraminidase , gel electrophoresis , cell , membrane , microbiology and biotechnology , biology , enzyme
Cell‐surface glycoproteins of rat liver sinusoidal plasma membranes from control and regenerating livers were studied. The glycoproteins were labeled using specific methods for sialic acid (NAIO 4 /NaB 3 H 4 ) and galactosyl/ N ‐acetyl galctosaminyl residues (galactose oxidase/NaB 3 H 4 and neuraminidase‐galactose oxidase (NaB 3 H 4 ) and the solubilized proteins were analyzed by gel electrophoresis. The patterns obtained with regenerating livers were quantitatively different from controls. This shows that cell surface glycoproteins change during liver regeneration.