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Involvement of plastoquinone and lipids in electron transport reactions mediated by the cytochrome b 6 ‐ f complex isolated from spinach
Author(s) -
Chain Richard K.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81095-4
Subject(s) - plastoquinone , chemistry , cytochrome , phosphatidylglycerol , cytochrome b6f complex , spinach , oxidoreductase , phosphatidylcholine , electron transport chain , biochemistry , biophysics , photosystem i , phospholipid , photosystem ii , biology , thylakoid , enzyme , photosynthesis , membrane , chloroplast , gene
The isolation of a cytochrome b 6 ‐ f complex from spinach, which is depleted of plastoquinone (and lipid), is reported. The depleted complex no longer functions as a plastoquinol‐plastocyanin oxidoreductase but can be reconstituted with plastoquinone and exogenous lipids. The lipid classes digalactosyldiacylglycerol, phosphatidylglycerol and phosphatidylcholine were active in reconstitution while monogalactosyldiacylglycerol and sulfoquinovosyldiacylglycerol were not. Neither plastoquinone nor lipid alone fully reconstitutes electron transport in the depleted complex. Saturation of plastoquinol‐plastocyanin oxidoreductase activity in the depleted complex occurs at 1 plastoquinone per cytochrome f .