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Kinetics of the H + ‐ATPase in chromatophores from Rhodospirillum rubrum
Author(s) -
Boork Jeff,
Strid Ake,
Baltscheffsky Margareta
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81093-0
Subject(s) - rhodospirillum rubrum , reaction rate constant , kinetics , chemistry , chromatophore , atpase , photochemistry , biophysics , biochemistry , biology , enzyme , physics , quantum mechanics , fishery
The effect of the electrical potential on the H + ‐ATPase of Rhodospirillum rubrum is examined. It is shown that the forward reaction rate (ATP synthesis) is increased by a factor of 10 during illumination while the reversed rate is only slightly decreased. This indicates that the electrical potential across the membrane affects the rate constants mainly by increasing the forward rate constants rather than decreasing the reversed rate constants in order to go from net hydrolysis to net synthesis.