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The Cl − effect on photosynthetic oxygen evolution: interaction of Cl − with 18‐kDa, 24‐kDa and 33‐kDa proteins
Author(s) -
Miyao Mitsue,
Murata Norio
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81091-7
Subject(s) - oxygen evolution , photosystem ii , oxygen , chemistry , photosynthesis , spinach , biochemistry , molecular oxygen , molecular mass , biophysics , biology , enzyme , electrochemistry , organic chemistry , electrode
The interaction of Cl − with the extrinsic proteins of 18 kDa, 24 kDa and 33 kDa in the photosynthetic oxygen‐evolution complex was studied by comparing spinach photosystem II particles of different protein compositions. The 33‐kDa protein decreased the Cl − concentration optimum for oxygen evolution from 150 to 30 mM, and the 24‐kDa protein decreased it from 30 to 10 mM. The 18‐kDa protein did not change the optimum Cl − concentration, but sustained oxygen evolution at Cl − concentrations lower than 3 mM. The presence of the 24‐kDa and 18‐kDa proteins, but not each protein alone, markedly suppressed inactivation of oxygen evolution at a very low Cl − concentration and its restoration by readdition of Cl − .