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Cyclic AMP stimulates dephosphorylation of specific proteins in intact S49 mouse lymphoma cells
Author(s) -
Kiss Zoltán,
Steinberg Robert A.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81072-3
Subject(s) - dephosphorylation , microbiology and biotechnology , chemistry , phosphorylation , lymphoma , biology , phosphatase , immunology
Two‐dimensional gel electrophoresis of proteins labeled with 32 P 1 in S49 mouse lymphoma cells revealed five phosphoproteins that were rapidly and reversibly dephosphorylated in response to elevation of cyclic AMP (cAMP). Under basal conditions, labeling of at least two of these proteins was limited by slow turnover of protein‐bound phosphate. The rapid cAMP‐mediated dephosphorylation of these species was attributable, therefore, to stimulation of a specific phosphoprotein phosphatase.