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Purification of rabbit tumor necrosis factor
Author(s) -
Abe Shigeru,
Gatanaga Tetsuya,
Yamazaki Masatoshi,
Soma Gen'ichiro,
Mizuno Den'ichi
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81071-1
Subject(s) - sephadex , tumor necrosis factor alpha , rabbit (cipher) , fractionation , chromatography , chemistry , sepharose , ammonium sulfate , lagomorpha , yield (engineering) , amino acid , necrosis , microbiology and biotechnology , biochemistry , biology , immunology , enzyme , endocrinology , statistics , materials science , mathematics , genetics , metallurgy
Rabbit tumor necrosis factor (TNF) was purified and shown by SDS‐PAGE to be a single protein of 18 kDa. TNF in 355 ml rabbit serum was precipitated with ammonium sulfate, and purified by repeated DEAE‐Sephadex and Sephacryl S‐200 chromatographies, and the final fractionation on Blue‐Sepharose 6B. By this procedure its yield was 22% and its specific activity was 2.4 × 10 7 U/mg protein. The sequence of the N‐terminal 20 amino acids was determined.