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Rapid proton release accompanying photosynthetic electron transport in intact cells of Rhodopseudomonas capsulata
Author(s) -
Taylor M.A.,
Jackson J.B.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81060-7
Subject(s) - antimycin a , biophysics , proton , chemistry , electron transport chain , photosynthesis , photosynthetic reaction centre , photochemistry , rhodopseudomonas , biochemistry , biology , physics , quantum mechanics
The release of protons from intact cells of Rhodopseudomonas capsulata after either 4μs flashes or during brief periods of continuous illumination has been measured with the indicator, cresol red. The half‐time for H + ‐release after a flash was 35 ms and the extent, 1H + per 134 bacteriochlorophyll. Myxothiazol completely inhibited the flash‐induced H + ‐release and antimycin A reduced it by 37%. The proton‐releasing reaction is discussed with reference to the protonmotive Q‐cycle. During continuous illumination the rapid phase of H + release is followed by a lag and then by another period of acidification, suggesting that other protolytic reactions may be in operation.