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Redox‐dependent activation of 5'‐nucleotidase in rat liver plasma membranes
Author(s) -
Tarakhovsky Alexander M.,
Umansky Victor J.,
Shlyakhovenko Vladimir A.,
Balitsky Konstantin P.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81051-6
Subject(s) - 5' nucleotidase , membrane , chemistry , nad+ kinase , biochemistry , rotenone , redox , enzyme , nucleotidase , cyanide , potassium cyanide , dehydrogenase , mitochondrion , inorganic chemistry
Addition of NADH, but not NAD + or NADPH, to rat liver plasma membranes resulted in the increase of their 5'‐nucleotidase activity. NADH‐dependent activation of 5'‐nucleotidase was significantly suppressed by atebrine, an inhibitor Of NADH dehydrogenase of plasma membranes, and completely abolished by 2,4‐dinitrophenol (2 × 10 −4 M) and Triton X‐100 (2%). Inhibitors of electron transfer in the mitochondrial respiratory chain, rotenone and potassium cyanide, failed to affect 5'‐nucleotidase activity in both the presence and absence of NADH. The data obtained give reasons to suggest a redox‐dependent mechanism of 5'‐nucleotidase activation in rat liver plasma membranes.