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Detection of the tripeptide Tyr‐Gly‐Gly, a putative enkephalin metabolite in brain, using a sensitive radioimmunoassay
Author(s) -
Llorens-Cortes Catherine,
Schwartz Jean-Charles,
Gros Claude
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81049-8
Subject(s) - tripeptide , radioimmunoassay , immunogen , metabolite , chemistry , chromatography , high performance liquid chromatography , enkephalin , biochemistry , peptide , biology , antibody , receptor , opioid , immunology , monoclonal antibody
A sensitive and specific radioimmunoassay has been developed for YOG. The tripeptide was previously derivatised with p ‐benzoquinone to prepare the immunogen and the 125 tracer as well as in samples submitted to the RIA. The sensitivity is about 1 nM as compared with 8000 nM for underivatised YGG. Measurable amounts of endogenous YGG immunoreactivity, co‐eluting in HPLC with authentic YGG, were detected in mouse striatal extracts.