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E. coli Ada regulatory protein repairs the S p diastereoisomer of alkylated DNA
Author(s) -
Hamblin Michael R.,
Potter Barry V.L.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81047-4
Subject(s) - diastereomer , alkylation , dna , chemistry , nuclear magnetic resonance spectroscopy , absolute configuration , stereochemistry , biochemistry , catalysis
Using HPLC and 31 P NMR spectroscopy on a chemically synthesized asymmetric mixture of the diastereoisomers of thymidyl(3' → 5')thymidyl‐ O ‐methyl phosphate absolute configuration has been correlated with Chromatographic mobility. The methyl phosphotriester system in alkylated DNA which is repaired by the Ada regulatory protein of E. coli has consequently been established to possess the S p configuration.