Premium
Rapid exchange of histones H2A and H2B in sea urchin embryo chromatin
Author(s) -
Schwager S.,
Retief J.D.,
de Groot P.,
von Holt C.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81045-0
Subject(s) - blastula , sea urchin , micrococcal nuclease , histone , chromatin , nucleosome , chemistry , microbiology and biotechnology , embryo , biology , biophysics , gastrulation , biochemistry , embryogenesis , dna
Chromatin from sea urchin blastula and gastrula was partially digested with micrococcal nuclease and separated electrophoretically in two dimensions into various nucleosome fractions and their component histones. After labelling of the embryos with radioactive lysine the various isohistones of the H2A and H2B group in all nucleosome fractions had incorporated approximately twice as much the radioactivity as the histones H2 and H4. The results are interpreted in terms of a labile nucleosome structure during transcription.