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Antibodies directed against guanylic acid and guanosine are nucleolus specific probes
Author(s) -
Iswari S.,
Krishnamurthy D.S.,
Jacob T.M.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81043-7
Subject(s) - guanosine , nucleolus , metaphase , guanosine monophosphate , chemistry , microbiology and biotechnology , interphase , biochemistry , immunofluorescence , antibody , rna , biology , nucleotide , genetics , chromosome , cytoplasm , gene
Purified antibodies against guanylic acid and guanosine binding to RNA at guanosine residues were used to probe human lymphocyte preparations by indirect immunofluorescence. Neither antibody gave any banding pattern with metaphase chromosomes but both showed binding to specific sites in the interphase nuclei. Evidence presented indicates that these sites are guanosine residues on rDNA transcripts at the nucleolar organizer regions.