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A small phospholipase inhibitory factor released by cultured cell lines
Author(s) -
Wood John N.,
Coote Paul R.,
Salmon John,
Rhodes John
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81023-1
Subject(s) - zymosan , arachidonic acid , cell culture , ionophore , phospholipase a2 , chemistry , phospholipase a , calcium , biochemistry , phospholipase , microbiology and biotechnology , biology , in vitro , enzyme , membrane , organic chemistry , genetics
Cells of the mouse macrophage‐like cell line RAW 264 release a dialysable inhibitor of phospholipase activity into their culture medium. This inhibitor can be detected in saline solution, Hanks solution and a variety of tissue culture media in the presence or absence of serum. The inhibitor is stable at 4°C, unaffected by trypsin, nucleases, or boiling, and partially extractable with chloroform/methanol. The release of both arachidonic acid and prostaglandins from mouse macrophages or human monocytes is inhibited by this material. A variety of other cell types release the inhibitor, which is effective against stimulation of arachidonic acid release from cultured macrophages by zymosan, serum, immune complexes and the calcium ionophore A23187.