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Glucuronidation of bile acids in human liver, intestine and kidney
Author(s) -
Parquet M.,
Pessah M.,
Sacquet E.,
Salvat C.,
Raizman A.,
Infante R.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81020-6
Subject(s) - glucuronidation , kidney , chemistry , biochemistry , medicine , gastroenterology , microsome , enzyme
The activities of UDP‐glucuronyl transferase(s) in homogenates and microsomal preparations of human liver, kidney and intestine were tested with hyodeoxycholic acid (HDC). The various kinetic parameters of the UDC‐glucuronidation were determined from time course experiments. In both liver and kidney preparations, HDC underwent a very active metabolic transformation: liver K m = 78 μM, V max = 3.3 nmol·min −1 mg −1 protein; kidney K m = 186 μM, V max = 9.9 nmol·min −1. mg −1 protein. To our knowledge this is the first observation of both an extensive and comparable bile acid glucuronidation occurring in renal and hepatic tissues.