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Structure and biological activity of polysomes stained with Coomassie blue
Author(s) -
Murthy M.R.V.,
Han.,
Bharucha A.D.,
Charbonneau R.,
Viallard J.-L.,
Dastugue B.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81008-5
Subject(s) - coomassie brilliant blue , polysome , chemistry , biochemistry , chromatography , staining , biology , ribosome , rna , genetics , gene
Polysomes prestained with Coomassie blue were fractionated on sucrose density gradients giving rise to visible bands corresponding to different size classes of aggregates. Coomassie blue staining enhanced the capacities of brain and liver polysomes to synthesize proteins in vitro, including the synthesis of neuron‐specific enolase. This positive action of the dye was restricted to polysomes and was not manifested when mRNAs isolated from prestained polysomes were tested in in vitro translation or reverse transcription, indicating that the action of the dye consists in stabilization of polysomal structure.