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Revised amino acid sequence of the B‐chain of ricin D due to loss of tryptophan in the cyanogen bromide cleavage
Author(s) -
Araki Tomohiro,
Funatsu Gunki
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)81006-1
Subject(s) - cyanogen bromide , edman degradation , ricin , peptide sequence , amino acid , chemistry , cleavage (geology) , sequence (biology) , complete sequence , tryptophan , stereochemistry , biochemistry , biology , paleontology , genome , fracture (geology) , toxin , gene
The complete amino acid sequence of the ricin D B‐chain was redetermined using the DABITC/PITC double‐coupling method. By comparing the presently established amino add sequence with the sequence reported earlier which was determined by the manual Edman degradation technique, it was found that the identification of 26 amino acid residues had to be revised. Furthermore, the ricin D B‐chain was shown to consist of 262 amino acid residues rather than 260 as previously described. In addition, based on the new amino acid sequence, the sequence homology between the N‐ and C‐terminal domain of this chain was found to be 31%.