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Single channel recordings obtained from basolateral membranes of isolated rabbit enterocytes
Author(s) -
Sepúlveda F.V.,
Mason W.T.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80999-6
Subject(s) - epithelial polarity , biophysics , membrane , rabbit (cipher) , chemistry , patch clamp , potassium channel , incubation , enterocyte , hyaluronidase , ion channel , small intestine , anatomy , microbiology and biotechnology , biochemistry , biology , enzyme , statistics , receptor , mathematics
Epithelial cells isolated by hyaluronidase incubation from rabbit small intestine were used to explore the presence of ionic channels by the patch‐clamp method. Recordings were made from cell‐attached or excised patches of basolateral membrane. Evidence was obtained for the presence of at least two kinds of channels conducting potassium currents. One of these can be shown to be activated by Ca 2+ .