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Quantitation of chordin in developing Huso huso embryos and larvae by radioimmunoassay
Author(s) -
Preobrazhensky A.A.,
Glinka A.V.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80998-4
Subject(s) - chordin , radioimmunoassay , sturgeon , huso , biology , fishery , embryo , embryogenesis , fish <actinopterygii> , endocrinology , gastrulation
Chordin is a protein discovered in the notochord cells of the representatives of Acipenseridae; giant sturgeon, stellate sturgeon and sterlet. Some characteristics of the purified chordin preparation which justify its use in radioimmunoassay are described. A sensitive competitive‐binding double‐antibody radioimmunoassay for chordin is described by which its content in the extracts from giant sturgeon embryos and larvae has been measured. It is shown that chordin biosynthesis started in the embryos from stage 32.