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Interaction of clathrin with liposomes: pH‐dependent fusion of phospholipid membranes induced by clathrin
Author(s) -
Hong Keelung,
Yoshimura Tetsuro,
Papahadjopoulos Demetrios
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80985-6
Subject(s) - clathrin , liposome , vesicle , phosphatidylcholine , phospholipid , phosphatidylserine , chemistry , lipid bilayer fusion , clathrin adaptor proteins , biophysics , bilayer , membrane , endocytosis , lipid bilayer , biochemistry , biology , receptor
Clathrin, dissociated from coated vesicles of bovine brain and purified by gel chromatography, was found to interact with the lipid bilayer as shown by the spontaneous release of encapsulated fluorescent dye in liposome. Clathrin‐induced dye release was enhanced at acidic pH in phosphatidylserine‐containing vesicles. A strong correlation between dye release and fusion of liposomes was observed. In general, when there was a fast release of encapsulated dye induced by clathrin, a pH‐dependent, clathrin‐induced fusion was observed. Clathrin did not induce either dye release or fusion of egg phosphatidylcholine liposomes. The self‐association of clathrin at low pH diminished the fusogenic activity. Fusion induced by clathrin at low pH c̀ould be stopped at pH above 5.0 and resumed by lowering the pH below 5.0. This suggests that the interaction of clathrin with phospholipid membranes can be regulated by pH.