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Cardiolipin vesicles can accommodate cholesterol up to 0.80 mole fraction, i.e. one molecule per cardiolipin fatty acid chain
Author(s) -
Gallay Jacques,
de Foresta Béatrice,
Vincent Michel
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80984-4
Subject(s) - cardiolipin , chemistry , vesicle , mole , biochemistry , fatty acid , mole fraction , membrane , phospholipid
Cardiolipin‐cholesterol interactions were studied by time‐resolved fluorescence spectroscopy with 1,6‐diphenyl‐1, 3,5‐hexatriene as probe. The residual anisotropy parameter, r ∞ (reflecting the fatty acid chain packing), was measured in the liquid crystalline phase as a function of cholesterol addition. Two main results are reported: (i) a slight increase of the order parameter, S , computed from the r ∞ value as S = ( r ∞ / r 0 ) , in the physiological concentration range of cholesterol; (ii) a sharp enhancement of S from a cholesterol mole fraction ( X̄ chl ) of 0.20 and up to X̄ chl of 0.80. This is in contrast to unsaturated kcithin systems for which a continuous increase of the order parameter was monitored, culminating at X̄ chl = 0.50, the wellknown maximum level of incorporation of cholesterol into lecithin model membranes.