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Purification of antineoplastic factor from eggs of a sea hare
Author(s) -
Yamazaki Masatoshi,
Kisugi Jun,
Kimura Katsuya,
Kamiya Hisao,
Mizuno Den'ichi
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80926-1
Subject(s) - cytolysis , lysis , size exclusion chromatography , aplysia , sea urchin , biology , microbiology and biotechnology , chemistry , biochemistry , chromatography , enzyme , cytotoxicity , in vitro , ecology , evolutionary biology
An antineoplastic factor inducing tumor lysis was purified to apparent homogeneity from the supernatant of a homogenate of eggs of the sea hare Aplysia kwodai . On gel filtration, the purified substance gave a single band of material of 250 kDa containing 3 different subunits and this band coincided with cytolytic activity. The purified factor was half‐maximally active at 10–60 ng protein/ml and lysed all tumor cells tested, but not normal cells. Antineoplastic factor Tumor lysis Sea hare Aplysia Cytolysis