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Inhibition by forskolin of cytosolic calcium rise, shape change and aggregation in quin2‐loaded human platelets
Author(s) -
Sage S.O.,
Rink T.J.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80890-5
Subject(s) - forskolin , chemistry , cyclase , platelet , ionophore , adenylate kinase , calcium , endocrinology , medicine , thrombin , cytosol , biophysics , biochemistry , enzyme , in vitro , biology , organic chemistry
The adenylate cyclase stimulator forskolin was used to study the inhibitory effects of elevated cAMP on the activation of washed human platelets loaded with the fluorescent Ca 2+ indicator quinl. In the presence of 10 μM isobutylmethylxanthine forskolin inhibited rises in [Ca 2+ ] i evoked by thrombin and platelet‐activating factor (PAF) due to both Ca 2+ influx and release from internal stores with similar potency. Aggregation evoked by thrombin and PAF was suppressed whilst partial shape‐change persisted, even in the absence of a measurable rise in [Ca 2+ ] i . Forskolin did not affect the rise in [Ca 2+ i evoked by Ca 2+ ionophore; aggregation was suppressed but shape‐change persisted.