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Comparison of genomes of closely related phages ø29, ø15 and PZA using a rapid method of parallel physical mapping
Author(s) -
Hostomský Zdeněk,
Pačes Václav,
Zadražil Stanislav
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80887-5
Subject(s) - ecori , genome , bacillus subtilis , restriction enzyme , hindiii , physical mapping , dna , biology , genetics , restriction map , gene , bacteria , plasmid , chromosome , gene mapping
The DNAs of phages ø29, ø15 and PZA of Bacillus subtilis were analysed with restriction enzymes Eco RI, Hpa I and Hin dIII. A method was used which permits parallel physical mapping of all three phages, from both ends of their linear genomes. The method is based on transfer of partially digested DNA to DBM paper and sequential hybridization with labelled terminal fragments. It follows from the comparison of the physical maps that phages ø29, ø15 and PZA are closely related and that they probably have arisen from a common ancestor by accumulation of point mutations.