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Separation of phosphorylated from non‐phosphorylated LHCP polypeptides by two‐dimensional electrophoresis
Author(s) -
Rémy R.,
Ambard-Bretteville F.,
Dubertret G.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80871-1
Subject(s) - isoelectric focusing , electrophoresis , chaps , phosphorylation , chromatography , isoelectric point , gel electrophoresis , chemistry , biochemistry , free flow electrophoresis , microbiology and biotechnology , biology , polyacrylamide gel electrophoresis , gel electrophoresis of proteins , enzyme
‘In vitro’ phosphorylated thylakoid polypeptides were studied by means of different electrophoretic techniques. A highly resolving two‐dimensional electrophoresis method, recently developed in the laboratory using CHAPS and SDS as detergent for electrofocusing, allows the separation of each of the LHCP apoproteins into several molecular species. Those having more acidic isoelectric points correspond to the phosphorylated forms.