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Oligonucleotide‐directed mutagenesis of the Rhizobium japonicum nifH promoter
Author(s) -
Kaluza Klaus,
Alvarez-Morales Ariel,
Hennecke Hauke
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80870-x
Subject(s) - transversion , site directed mutagenesis , mutagenesis , promoter , biology , rhizobium , genetics , microbiology and biotechnology , nitrogenase , gene , mutation , mutant , gene expression , bacteria , nitrogen fixation
A deoxyguanosine (G) to deoxythymidine (T) transversion was introduced, by site‐specific mutagenesis, at position −25 of the nif consensus promoter sequence. It resulted in a 92% decrease of promoter activity when tested in an enterobacterial NtrA + NifA + system that was known to activate Rhizobium japonicum nitrogenase promoters. Two other mutations, in regions that were previously speculated to be of potential importance for nifH expression [(1984) Mol. Gen. Genet. 196, 35‐42; (1984) J. Mol. Appl. Genet. 2, 395‐405], were without severe effect.