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Periodic heat production by oscillating glycolysis in a cytoplasmic medium extracted from yeast
Author(s) -
Plesser T.,
Müller S.C.,
Hess B.,
Lamprecht I.,
Schaarschmidt B.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80838-3
Subject(s) - yeast , calorimeter (particle physics) , cytoplasm , saccharomyces cerevisiae , calorimetry , glycolysis , chemistry , production rate , biochemistry , enthalpy , thermodynamics , biophysics , metabolism , biology , physics , industrial engineering , detector , optics , engineering
The rate of heat production in a periodically glycolysing cell‐free cytoplasmic medium extracted from yeast Saccharomyces cerevisiae is measured with a batch calorimeter. The rate exhibits periodic variations of approx. 10% of the average heat production rate of about 54 per minute. From this rate and the enthalpy change fro glycolysis a glucose degradation rate of 0.43 is calculated. The value fits into the ‘oscillatory window’ determined by a glucose injection technique.